RESUMO
Here we describe the detection and characterisation of three isolates of vancomycin-resistant VanB-type Enterococcus faecalis. Sequence analysis suggested that these isolates harboured the vanB1 gene. The isolates were susceptible to the majority of antimicrobial agents tested, with the exception of chloramphenicol, erythromycin and vancomycin, and showed distinct profiles of high-level resistance to aminoglycosides. Analysis of the clonal relatedness of the vanB E. faecalis isolates showed similar pulsed-field gel electrophoresis profiles. To our knowledge, this is the first report of the occurrence of enterococcal strains carrying vanB genes in Brazil.
Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Infecção Hospitalar/microbiologia , Enterococcus faecalis/genética , Infecções por Bactérias Gram-Positivas/microbiologia , Resistência a Vancomicina/genética , Brasil , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Eletroforese em Gel de Campo Pulsado , Enterococcus faecalis/efeitos dos fármacos , HumanosRESUMO
Here we describe the detection and characterisation of three isolates of vancomycin-resistant VanB-type Enterococcus faecalis. Sequence analysis suggested that these isolates harboured the vanB1 gene. The isolates were susceptible to the majority of antimicrobial agents tested, with the exception of chloramphenicol, erythromycin and vancomycin, and showed distinct profiles of high-level resistance to aminoglycosides. Analysis of the clonal relatedness of the vanB E. faecalis isolates showed similar pulsed-field gel electrophoresis profiles. To our knowledge, this is the first report of the occurrence of enterococcal strains carrying vanB genes in Brazil.
Assuntos
Humanos , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Infecção Hospitalar/microbiologia , Enterococcus faecalis/genética , Infecções por Bactérias Gram-Positivas/microbiologia , Resistência a Vancomicina/genética , Brasil , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Eletroforese em Gel de Campo Pulsado , Enterococcus faecalis/efeitos dos fármacosRESUMO
Os microrganismos do genero Lactococcus apresentam caracteristicas fenotipicas semelhantes as do genero Enterococcus podendo ser erroneamente identificados como tal no laboratorio clinico. Durante um estudo epidemiologico visando a deteccao de enterococos resistentes a vancomicina, foram avaliadas 155 amostras, inicialmente suspeitas de pertencerem ao genero Enterococcus, isoladas da microbiota intestinal e de diversos materiais clinicos oriundos de pacientes atendidos em Instituicoes de Saude do Municipio de Niteroi, no periodo entre janeiro de 2005 e janeiro de 2006. Duas amostras, que apresentaram caracteristicas fisiologicas naototalmente compativeis com o genero Enterococcus, foram identificadas como Lactococcus garvieae atraves da utilizacao de testes adicionaiscomo a analise do perfil proteico total atraves de eletroforese em gel de poliacrilamida contendo dodecil sulfato de sodio. Esta metodologia pode ser uma alternativa para identificar corretamente L. garvieae visto que e uma tecnica relativamente simples e demenor custo, quando comparada aos testes baseados na analise do DNA.
The members of the genus Lactococcus have phenotypic characteristics that resemble those of the genus Enterococcus and therefore can be erroneously identified in the clinical laboratory. During an epidemiological study aiming the detection of vancomycin-resistant enterococci, were evaluated 155 isolates, initially suspected of belonging to the genus Enterococcus, recovery from the intestinal microflora and different clinical materials obtained from patients at Health Institutions of Niteroi city, in the period between January 2005 and January 2006. Two isolates, which showed physiological characteristics not fully compatible with the genusEnterococcus, 2006. Two isolates, which showed physiological characteristics not fully compatible with the genus Enterococcus, were identified as Lactococcus garvieae by using additional physiological tests and analysis of total protein profiles by polyacrylamide gel electrophoresis, containing sodium dodecyl sulfate. This is an alternative methodology to correctly identify L. garvieae provided it is relatively easy to perform and of lower cost when compared to DNA based tests.
